Electroanatomical mapping of the stomach with simultaneous biomagnetic measurements.

Gastric motility is coordinated by bioelectric slow waves (SWs) and dysrhythmic SW activity has been linked with motility disorders. Magnetogastrography (MGG) is the non-invasive measurement of the biomagnetic fields generated by SWs. Dysrhythmia identification using MGG is currently challenging because source models are not well developed and the impact of anatomical variation is not well understood. A novel method for the quantitative spatial co-registration of serosal SW potentials, MGG, and geometric models of anatomical structures was developed and performed on two anesthetized pigs to verify feasibility. Electrode arrays were localized using electromagnetic transmitting coils. Coil localization error for the volume where the stomach is normally located under the sensor array was assessed in a benchtop experiment, and mean error was 4.2±2.3mm and 3.6±3.3° for a coil orientation parallel to the sensor array and 6.2±5.7mm and 4.5±7.0° for a perpendicular coil orientation. Stomach geometries were reconstructed by fitting a generic stomach to up to 19 localization coils, and SW activation maps were mapped onto the reconstructed geometries using the registered positions of 128 electrodes. Normal proximal-to-distal and ectopic SW propagation patterns were recorded from the serosa and compared against the simultaneous MGG measurements. Correlations between the center-of-gravity of normalized MGG and the mean position of SW activity on the serosa were 0.36 and 0.85 for the ectopic and normal propagation patterns along the proximal-distal stomach axis, respectively. This study presents the first feasible method for the spatial co-registration of MGG, serosal SW measurements, and subject-specific anatomy. This is a significant advancement because these data enable the development and validation of novel non-invasive gastric source characterization methods.

Stomach Geometry Reconstruction Using Serosal Transmitting Coils and Magnetic Source Localization.

OBJECTIVE: Bioelectric slow waves (SWs) are a key regulator of gastrointestinal motility, and disordered SW activity has been linked to motility disorders. There is currently a lack of practical options for the acquisition of the 3D stomach geometry during research studies when medical imaging is challenging. Accurately recording the geometry of the stomach and co-registering electrode and sensor positions would provide context for in-vivo studies and aid the development of non-invasive methods of gastric SW assessment. METHODS: A stomach geometry reconstruction method based on the localization of transmitting coils placed on the gastric serosa was developed. The positions and orientations of the coils, which represented boundary points and surface-normal vectors, were estimated using a magnetic source localization algorithm. Coil localization results were then used to generate surface models. The reconstruction method was evaluated against four 3D-printed anatomically realistic human stomach models and applied in a proof of concept in-vivo pig study. RESULTS: Over ten repeated reconstructions, average Hausdorff distance and average surface-normal vector error values were 4.7 ±0.2 mm and 18.7 ±0.7° for the whole stomach, and 3.6 ±0.2 mm and 14.6 ±0.6° for the corpus. Furthermore, mean intra-array localization error was 1.4 ±1.1 mm for the benchtop experiment and 1.7 ±1.6 mm in-vivo. CONCLUSION AND SIGNIFICANCE: Results demonstrated that the proposed reconstruction method is accurate and feasible. The stomach models generated by this method, when co-registered with electrode and sensor positions, could enable the investigation and validation of novel inverse analysis techniques.

Multichannel magnetogastrogram: a clinical marker for pediatric chronic nausea.

Chronic nausea is a widespread functional disease in children with numerous comorbidities. High-resolution electrogastrogram (HR-EGG) has shown sufficient sensitivity as a noninvasive clinical marker to objectively detect distinct gastric slow wave properties in children with functional nausea. We hypothesized that the increased precision of magnetogastrogram (MGG) slow wave recordings could provide supplementary information not evident on HR-EGG. We evaluated simultaneous pre- and postprandial MGG and HR-EGG recordings in pediatric patients with chronic nausea and healthy asymptomatic subjects, while also measuring nausea intensity and nausea severity. We found significant reductions in postprandial dominant frequency and normogastric power, and higher levels of postprandial bradygastric power in patients with nausea in both MGG and HR-EGG. MGG also detected significantly lower preprandial normogastric power in patients. A significant difference in the mean preprandial gastric slow wave propagation direction was observed in patients as compared with controls in both MGG (control: 180 ± 61°, patient: 34 ±72°; P < 0.05) and HR-EGG (control: 240 ± 39°, patient: 180 ± 46°; P < 0.05). Patients also showed a significant change in the mean slow wave direction between pre- and postprandial periods in MGG (P < 0.05). No statistical differences were observed in propagation speed between healthy subjects and patients in either MGG or HR-EGG pre/postprandial periods. The use of MGG and/or HR-EGG represents an opportunity to assess noninvasively the effects of chronic nausea on gastric slow wave activity. MGG data may offer the opportunity for further refinement of the more portable and economical HR-EGG in future machine-learning approaches for functional nausea.NEW & NOTEWORTHY Pediatric chronic nausea is a difficult-to-measure subjective complaint that requires objective diagnosis, clinical assessment, and individualized treatment plans. Our study demonstrates that multichannel MGG used in conjunction with custom HR-EGG detects key pathological signatures of functional nausea in children. This quantifiable measure may allow more personalized diagnosis and treatment in addition to minimizing the cost and potential radiation associated with current diagnostic approaches.

Anatomically Constrained Gastric Slow Wave Localization using Biomagnetic Data.

Detection of dysrhythmic gastric slow wave (SW) activity could have significant clinical utility because dysrhyth-mias have been linked to gastric motility disorders. The elec-trogastrogram (EGG) and magnetogastrogram (MGG) enable the non-invasive assessment of SW activity, but most analysis methods can only resolve frequency and velocity. Improved characterization of dysrhythmic propagation patterns from non-invasive measurements is important for the diagnosis of motility disorders and could allow early treatment stratification. In this study, we demonstrate the use of a penalized linear regression framework to localize SW events on the longitudinal stomach axis using simulated MGG data. Priors relating to spatial sparsity, the organization of wavefronts into complete circumferential rings, and the local distribution of depolar-ization and repolarization phases were used to constrain the inverse solution. This method was applied to MGG computed for a single wavefront case and a multiple wavefront case that were constructed from simulated 3 cycle-per-minute normal SW activity. Propagation patterns along the longitudinal stomach axis were identifiable from reconstructed SW activity for both cases. Localization error was 5.7 ± 0.1 mm and 7.7 ± 0.1 mm for each respective case within the distal stomach when the signal-to-noise ratio was 10 dB. Results indicate that penalized linear regression can successfully localize SW events provided the 3D geometry of the stomach and torso were acquired. Clinical Relevance- This method could help to improve the efficiency and accuracy of diagnosing gastric motility disorders from non-invasive measurements.

Characterizing Spatial Signatures of Gastric Electrical Activity Using Biomagnetic Source Localization.

BACKGROUND: The motility patterns in the gastrointestinal tract are regulated, in part, by bioelectrical events known as slow waves (SWs). Understanding temporal and spatial features of gastric SWs can help reveal the underlying causes of functional motility disorders. OBJECTIVE: This study investigated the ability of source localization techniques to characterize the spatial signatures of SW activity using simulated and experimental magnetogastrography data. METHODS: Two SW propagation patterns (antegrade and retrograde) with two rhythms (normogastric and bradygastric) were used to simulate magnetic fields using 4 anatomically realistic stomach and torso geometries. Source localization was performed utilizing the equivalent current dipole (ECD) and the equivalent magnetic dipole (EMD) models. RESULTS: In the normogastric simulations when compared with the SW activity, the EMD model was capable of identifying the SW propagation in the lateral, antero-posterior, and supero-inferior axes with the median correlation coefficients of 0.66, 0.53, and 0.83, respectively, whereas the ECD model produced lower correlation scores (median: 0.52, 0.44, and 0.44). Moreover, the EMD model resulted in distinct and opposite spatial signatures for the antegrade and retrograde propagation. Similarly, when experimental data was used, the EMD model revealed antegrade-like signatures where the propagation was mostly towards the third quadrant in the supero-inferior (preprandial: 49%, postprandial: 35%) and antero-posterior (preprandial: 49%, postprandial: 50%) axes. CONCLUSION AND SIGNIFICANCE: The EMD model was able to identify and classify the spatial signatures of SW activities, which can help to inform the interpretation of non-invasive recordings of gastric SWs as a biomarker of functional motility disorders.

Automated Machine Learning Pipeline Framework for Classification of Pediatric Functional Nausea Using High-Resolution Electrogastrogram.

OBJECTIVE: Pediatric functional nausea is challenging for patients to manage and for clinicians to treat since it lacks objective diagnosis and assessment. A data-driven non-invasive diagnostic screening tool that distinguishes the electro-pathophysiology of pediatric functional nausea from healthy controls would be an invaluable aid to support clinical decision-making in diagnosis and management of patient treatment methodology. The purpose of this paper is to present an innovative approach for objectively classifying pediatric functional nausea using cutaneous high-resolution electrogastrogram data. METHODS: We present an Automated Electrogastrogram Data Analytics Pipeline framework and demonstrate its use in a 3x8 factorial design to identify an optimal classification model according to a defined objective function. Low-fidelity synthetic high-resolution electrogastrogram data were generated to validate outputs and determine SOBI-ICA noise reduction effectiveness. RESULTS: A 10 parameter support vector machine binary classifier with a radial basis function kernel was selected as the overall top-performing model from a pool of over 1000 alternatives via maximization of an objective function. This resulted in a 91.6% test ROC AUC score. CONCLUSION: Using an automated machine learning pipeline approach to process high-resolution electrogastrogram data allows for clinically significant objective classification of pediatric functional nausea. SIGNIFICANCE: To our knowledge, this is the first study to demonstrate clinically significant performance in the objective classification of pediatric nausea patients from healthy control subjects using experimental high-resolution electrogastrogram data. These results indicate a promising potential for high-resolution electrogastrography to serve as a data-driven screening tool for the objective diagnosis of pediatric functional nausea.

Sex Moderates the Relationship Between Nausea Severity and Heart Rate Variability in Adolescents and Young Adults.

BACKGROUND: Nausea is a common complaint among children and is particularly prevalent in children with functional abdominal pain (FAP), with nearly half of children with FAP also endorsing nausea. Dysfunction of the autonomic nervous system, which can be indexed by heart rate variability (HRV), leads to abnormalities in gastric electrical activity that are associated with GI symptoms. AIMS: To evaluate that relationship between nausea severity and HRV in adolescents and young adults with a history of FAP and to assess for sex differences. METHODS: Participants were pediatric patients with a diagnosis of FAP who were recruited from a pediatric GI clinic between 1993 and 2007 for a prospective study of the course of FAP. Study analyses focused on the cross-sectional relationship between HRV, indexed by standard deviation of the R-R interval (SDRRI) and high-frequency (HF) power, and nausea severity collected during a follow-up visit in late adolescence and young adulthood. RESULTS: Controlling for age and BMI, a significant nausea by sex interaction emerged for both SDRRI and HF power. Tests of conditional effects of nausea by sex showed that the inverse relation between nausea severity and both SDRRI and HF was significant for females but not for males. CONCLUSIONS: This is the first study to evaluate the relationship between nausea severity and HRV. Greater nausea severity was associated with lower HRV in females but not in males. Further validation of these results may provide insight into novel treatment approaches for females with nausea that target vagal tone.

Reconstruction of stomach geometry using magnetic source localization.

Routine diagnosis of gastric motility disorders represents a significant problem to current clinical practice. The non-invasive electrogastrogram (EGG) and magnetogastrogram (MGG) enable the assessment of gastric slow wave (SW) dysrhythmias that are associated with motility disorders. However, both modalities lack standardized methods for reliably detecting patterns of SW activity. Subject-specific anatomical information relating to the geometry of the stomach and its position within the torso have the potential to aid the development of relations between SWs and far-fields. In this study, we demonstrated the feasibility of using magnetic source localization to reconstruct the geometry of an anatomically realistic 3D stomach model. The magnetic fields produced by a small (6.35 × 6.35 mm) N35 neodymium magnet sequentially positioned at 64 positions were recorded by an array of 27 magnetometers. Finally, the magnetic dipole approximation and a particle swarm optimizer were used to estimate the position and orientation of the permanent magnet. Median position and orientation errors of 3.8 mm and 7.3° were achieved. The estimated positions were used to construct a surface mesh, and the Hausdorff Distance and Average Hausdorff Distance dissimilarity metrics for the reconstructed and ground-truth models were 11.6 mm and 2.4 mm, respectively. The results indicate that source localization using the magnetic dipole model can successfully reconstruct the geometry of the stomach.

The effect of chronic nausea on gastric slow wave spatiotemporal dynamics in children.

BACKGROUND: Chronic nausea in adolescents with functional gastrointestinal disorders is an increasingly reported but poorly understood symptom that negatively affects quality of life. Functional gastrointestinal disorders are known to correlate closely with slow wave rhythm disturbances. The ability to characterize gastric electrophysiologic perturbations in functional nausea patients could provide potential diagnostic and therapeutic tools for nausea patients. METHODS: We used high-resolution electrogastrograms (HR-EGG) to measure gastric slow wave parameters in pediatric chronic nausea patients and healthy subjects both pre- and postprandial. We computed the dominant frequency, percentage power distribution, gastric slow wave propagation direction, and speed from HR-EGG. KEY RESULTS: We observed significant differences in the dominant frequency and power distributed in normal and bradyarrhythmia frequency ranges when comparing patients and healthy subjects. Propagation patterns in healthy subjects were predominantly anterograde, while patients exhibited a variety of abnormalities including retrograde, anterograde, and disrupted patterns. There was a significant difference in the preprandial mean slow wave direction between healthy subjects (222° ± 22°) and patients (103° ± 66°; p Ë‚ 0.01), although the postprandial mean direction between healthy subjects and patients was similar (p = 0.73). No significant difference in slow wave propagation speed was found between patients and healthy subjects in either pre- (p = 0.21) or postprandial periods (p = 0.75). CONCLUSIONS AND INFERENCES: The spatiotemporal characterization of gastric slow wave activity using HR-EGG distinguishes symptomatic chronic nausea patients from healthy subjects. This characterization may in turn inform and direct clinical decision-making and lead to further insight into its pathophysiology.

Effects of magnetogastrography sensor configurations in tracking slow wave propagation.

Magnetogastrography (MGG) is a non-invasive method of assessing gastric slow waves (SWs) by recording the resultant magnetic fields. MGG can capture both SW frequency and propagation, and identify SW dysrhythmias that are associated with motility disorders. However, the impact of the restricted spatial coverage and sensor density on SW propagation tracking performance is unknown. This study simulated MGG using multiple anatomically specific torso geometries and two realistic SW propagation patterns to determine the effect of different sensor configurations on tracking SW propagation. The surface current density mapping and center-of-gravity tracking methods were used to compare four magnetometer array configurations: a reference system currently used in GI research and three hypothetical higher density and coverage arrays. SW propagation patterns identified with two hypothetical arrays (with coverage over at least the anterior of the torso) correlated significantly higher with simulated realistic 3 cycle-per-minute SW activity than the reference array (p = 0.016, p = 0.005). Furthermore, results indicated that most of the magnetic fields that contribute to the performance of SW propagation tracking were located on the anterior of the torso as further increasing the coverage did not significantly increase performance. A 30% decrease in sensor spacing within the same spatial coverage of the reference array also significantly increased correlation values by approximately 0.50 when the signal-to-noise ratio was 5 dB. This study provides evidence that higher density and coverage sensor layouts will improve the utility of MGG. Further work is required to investigate optimum sensor configurations across larger anatomical variations and other SW propagation patterns.

Computational Reconstruction of 3D Stomach Geometry using Magnetic Field Source Localization.

In this study, we investigated the feasibility of computationally reconstructing the 3D geometry of the stomach by performing source localization of the magnetic field (MF) induced from the stomach surface. Anatomically realistic stomach and torso models of a human participant, reconstructed from the CT images, were used in the computations. First, 128 coils with a radius of 5 mm were positioned on different locations on the stomach model. Next, MF at the sensor positions were computed using Bio-Savart law for the currents of 10 and 100 mA. Then, three noise levels were defined using the biomagnetic data recorded from the same participant and two additional sets of generated white-noise resulting in mean signal to noise ratios (SNR) of 20 and 10 dB. Finally, for each combination of the current and noise level, the magnetic dipole (MDP) approximation was performed to estimate coil positions. The performance of the source localization was assessed by computing the goodness of fit (GOF) values and the distance between the coil and the estimated MDP positions. We obtained GOF values over 98% for all coils and a mean localization error of 0.69±0.08 mm was achieved when 100 mA current was used to induce MF and only biomagnetic data was added. When additional white-noise was added, the GOF values decreased to 95% and the mean localization error increased to around 4 mm. A current of 10 mA was enough to localize the coil positions with a mean error around 8 mm even for the highest noise level we tested but for the few coils furthest from the body surface, the error was around 10 cm. The results indicate that source localization using the MDP approximation can successfully extract spatial information of the stomach.Clinical relevance-Extracting the spatial information of the stomach during the recording of the slow wave activity provides new insights in assessing gastric recordings and relating to disorders.

Simulation-based Analysis of Magnetogastrography Sensor Configurations for Characterizing Gastric Slow Wave Dysrhythmias.

The routine diagnosis of gastric motility disorders represents a significant problem to current clinical practice. Magnetogastrography (MGG) provides a non-invasive option for assessing gastric slow wave (SW) dysrhythmias that are associated with motility disorders. However, its ability to characterize SW propagation is impaired by the limited spatial coverage of existing superconducting quantum interference devices (SQUIDs). Recently developed optically-pumped magnetometers can potentially substitute SQUIDs and enable subject-specific MGG arrays with greater spatial coverage. This study developed simulations of gastric MGG to determine the distribution of the magnetic fields (MFs) generated by SWs above the torso, and investigated the impact of several realistic dysrhythmic patterns of propagation. The distribution of MFs was found to vary significantly for different patterns of SW propagation, with ectopic dysrhythmia displaying the greatest difference from normal. Notably, some important proportion of the MFs lay outside the coverage of an existing experimental SQUID array used in gastrointestinal research for some simulated SW propagation patterns, such as retrograde activity. Results suggest that MGG measurements should be made over the entire frontal face of the torso to capture all of the strongest MFs generated by SWs.Clinical relevance- This provides a guide for the placement of MGG sensors for the capture of both normal and dysrhythmic gastric slow wave propagation.

Magnetoenterography for the Detection of Partial Mesenteric Ischemia.

BACKGROUND: Acute mesenteric ischemia represents a life-threatening gastrointestinal condition. A noninvasive diagnostic modality that identifies mesenteric ischemia patients early in the disease process will enable early surgical intervention. Previous studies have identified significant changes in the small-bowel electrical slow-wave parameters during intestinal ischemia caused by total occlusion of the superior mesenteric artery. The purpose of this study was to use noninvasive biomagnetic techniques to assess functional physiological changes in intestinal slow waves in response to partial mesenteric ischemia. METHODS: We induced progressive intestinal ischemia in normal porcine subjects (n = 10) by slowly increasing the occlusion of the superior mesenteric artery at the following percentages of baseline flow: 50%, 75%, 90%, and 100% while simultaneous transabdominal magnetoenterogram (MENG) and serosal electromyogram (EMG) recordings were being obtained. RESULTS: A statistically significant serosal EMG amplitude decrease was observed at 100% occlusion compared with baseline, whereas no significant change was observed for MENG amplitude at any progressive occlusion levels. MENG recordings showed significant changes in the frequency and percentage of power distributed in bradyenteric and normoenteric frequency ranges at 50%, 75%, 90%, and 100% vessel occlusions. In serosal EMG recordings, a similar percent power distribution (PPD) effect was observed at 75%, 90%, and 100% occlusion levels. Serosal EMG showed a statistically significant increase in tachyenteric PPD at 90% and 100% occlusion. We observed significant increase in tachyenteric PPD only at the 100% occlusion level in MENG recordings. CONCLUSIONS: Ischemic changes in the intestinal slow wave can be detected early and noninvasively even with partial vascular occlusion. Our results suggest that noninvasive MENG may be useful for clinical diagnosis of partial mesenteric ischemia.

Source localization for gastric electrical activity using simulated magnetogastrographic data.

In this study, the use of magnetic dipole (MDP) approximation to localize the underlying source of magnetogastrographic (MGG) data was investigated. An anatomically realistic torso and a stomach model were used to simulate slow wave (SW) activities and magnetic fields (MFs). SW activity in the stomach was simulated using a grid-based finite element method. The SW activity at each time sample was represented by the dipoles generated for each element and MFs were computed from these dipoles including secondary sources in the torso. Gaussian noise was added to the MFs to represent experimental signal noise. MDP fitting was executed on the time samples of selected 2-second time frames, and goodness of fit (GOF) and the distance from the fitted MDP to the center of gravity (COG) of active dipoles were computed. Then, for each time frame, the spatial changes of COG and MDP positions in x-, y-, and z-directions and correlation scores were computed. Our results showed that MDP fitting was capable of identifying propagation patterns with mean correlation scores of 0.63 ± 0.30, 0.71 ± 0.19, and 0.81 ± 0.24 in x-, y-, and z-directions, respectively. The mean distance from COGs to the identified MDPs was 49±4 mm. The results were similar under the noise conditions as well. Our results suggest that source localization using MDP approximation can be useful to identify the propagation characteristics of SWs using MGG data.

Noninvasive Magnetogastrography Detects Erythromycin-Induced Effects on the Gastric Slow Wave.

OBJECTIVE: The prokinetic action of erythromycin is clinically useful under conditions associated with gastrointestinal hypomotility. Although erythromycin is known to affect the electrogastrogram, no studies have examined the effects that erythromycin has on gastric slow wave magnetic fields. METHODS: In this study, gastric slow wave activity was assessed simultaneously using noninvasive magnetogastrogram (MGG), electrogastrogram, and mucosal electromyogram recordings. Recordings were obtained for 30 min prior to and 60 min after intravenous administration of erythromycin at dosages of 3 and 6 mg/kg. RESULTS: MGG recordings showed significant changes in the percentage power distribution of gastric signal after infusion of both 3 and 6 mg/kg erythromycin at t = 1-5 min that persisted for t = 30-40 min after infusion. These changes agree with the changes observed in the electromyogram. We did not observe any statistically significant difference in MGG amplitude before or after injection of either 3 or 6 mg/kg erythromycin. Both 3 and 6 mg/kg erythromycin infusion showed retrograde propagation with a statistically significant decrease in slow wave propagation velocity 11-20 min after infusion. Propagation velocity started returning toward baseline values after approximately 21-30 min for the 3 mg/kg dosage and after 31-40 min for a dosage of 6 mg/kg. CONCLUSION: Our results showed that the magnetic signatures were sensitive to disruptions in normal slow wave activity induced by pharmacological and prokinetic agents such as erythromycin. SIGNIFICANCE: This study shows that repeatable noninvasive bio-electro-magnetic techniques can objectively characterize gastric dysrhythmias and may quantify treatment efficacy in patients with functional gastric disorders.